The Electron-dense Bodies of Mycobacterium phlei

Genetic transfer in Mycobacterium phlei.

Isolation from Mycobacterium Phlei

Mycobactin is the name suggested for a growth factor, present in acid-fast bacteria, which is essential for the growth of Mycobacterium johnei. A preliminary account ofthe work reported here has already been published (Francis, Madinaveitia, Macturk & Snow, 1949). Myco. johnei is an organism first observed by Johne & Frothingham (1895) in cases of chronic enteritis of cattle. It could not be cu...

Citrate formation in Mycobacterium phlei.

Although it is well known that citrate stimulates the growth of acid fast bacteria, the metabolic role of citrate is still obscure in this bacterial group. Washed suspensions of mycobacteria either fail to oxidize citrate or oxidize this substance slowly (Edson, 1951). On the other hand, clarified extracts of mycobacteria oxidize citrate to a-ketoglutarate (Geronimus, Gray, and Birkeland, 1949;...

The Appearance and General Properties of Free Radicals in Electron Transport Particles from Mycobacterium Phlei.

It has been demonstrated that the terminal electron transport pathway of Mycobacttium phlei resides in a particulate fraction of the organism (1). Evidence also has been obtained suggesting that a naturally occurring naphthoquinone is involved as an oxidation-reduction component in this pathway (2). Since a naphthoquinone may be involved in this system, the possibility exists that naphthosemiqu...

Orientation of the cell membrane in ghosts and electron transport particles of Mycobacterium phlei.

The chemical and enzymatic composition and the respiratory components of an intact membrane preparation, ghosts from Mycobacterium phlei, were compared to those of the electron transport particles. The ghosts were found to contain 5 to 7% of some of the soluble enzymes associated with the cytoplasm. In contrast, 97% of the total content of cytochromes b, cl + c, and a + a3 was found in the ghos...